List of Publications

2011

41. Volpato J.P., Mayotte N., Fossati E., Guerrero V., Sauvageau G., Pelletier J.N. (2011) Selectively weakened binding of methotrexate by human dihydrofolate reductase allows rapid ex vivo selection of mammalian cells. J Mol Recognit., 24 (2):188-98.

40. Banka S., Blom H.J., Walter J., Aziz M., Urquhart J., Clouthier C.M., Rice G.I., de Brouwer A.P., Hilton E., Vassallo G., Will A., Smith D.E., Smulders Y.M., Wevers R.A., Steinfeld R., Heales S., Crow Y.J., Pelletier J.N., Jones S., Newman W.G. (2011) Identification and characterization of an inborn error of metabolism caused by dihydrofolate reductase deficiency. Am J Hum Genet., 88 (2):216-25.

2010

39. Morin S., Clouthier C.M., Gobeil S., Pelletier J.N., Gagné S.M. (2010) Backbone resonance assignments of an artificially engineered TEM-1/PSE-4 Class A beta-lactamase chimera. Biomol NMR Assign., 4 (2):127-30

38. Bolduc O.R., Pelletier J.N., Masson J.F. (2010) SPR Biosensing in Crude Serum Using Ultralow Fouling Binary Patterned Peptide SAM. Anal Chem., 82 (9):3699-706.

2009

37. Gnaccarini C., Ben-Tahar W., Lubell W.D., Pelletier J.N., Keillor J.W. (2009) Fluorometric assay for tissue transglutaminase-mediated transamidation activity. Bioorg Med Chem., 17 (17): 6354-9.

36. Bolduc O.R., Clouthier C.M., Pelletier J.N., Masson J.F. (2009) Peptide self-assembled monolayers for label-free and unamplified surface plasmon resonance biosensing in crude cell lysate. Anal Chem., 81 (16): 6779-88.

35. Volpato J.P., Yachnin B.J., Blanchet J., Guerrero V., Poulin L., Fossati E., Berghuis A.M., Pelletier J.N. (2009) Multiple conformers in active site of human dihydrofolate reductase F31R/Q35E double mutant suggest structural basis for methotrexate resistance. J Biol Chem, 284 (30): 20079-89.

35. Pardin C., Roy I., Chica R.A., Bonneil E., Thibault P., Lubell W.D., Pelletier J.N., Keillor J.W. (2009) Photolabeling of tissue transglutaminase reveals the binding mode of potent cinnamoyl inhibitors. Biochemistry 48 (15): 3346-53.

34. Volpato J. P., and Pelletier J.N. (2009) Sequence and structural comparison of mammalian, bacterial and parasitic dihydrofolate reductases: cross-species mutational ‘hot-spots’ provide antifolate resistance. Drug Resistance Updates, 12 (1-2): 28-41.

33. De Wals P.-Y., Doucet N. and Pelletier J.N. (2009) High tolerance to simultaneous active-site mutations in TEM-1 beta-lactamase: Distinct mutational paths provide more generalized beta-lactam recognition. Protein Science, 18 (1): 147-160.

2008

32. Fossati, E. Volpato, J. P., Poulin, L., Guerrero, V., Dugas, D. A. et Pelletier, J. N. (2008) Two-tier in vivo and in vitro selection of active and methotrexate-resistant variants of human dihydrofolate reductase. Journal of Biomolecular Screening, 13(6) 504-514.

31. Pardin, C., Pelletier, J. N., Keillor, J. W. and Lubell, W. D. (2008) Cinnamoyl inhibitors of tissue transglutaminase. Journal of Organic Chemistry, 73, 5766-5775.

30. Keillor, J. W., Chica, R. A., Chabot, N., Vinci, V., Pardin, C., Fortin, E., Gillet, S.M.F.G., Nakano, Y., Kaartinen, M., Pelletier, J. N. and Lubell, W.D. (2008) The Bioorganic Chemistry of Transglutaminase: From Mechanism to Inhibition and Engineering. Canadian Journal of Chemistry,86, 271-276.

2007

29.J. N. Pelletier and M.G. Denault: "Protein library design and screening: Working out the probabilities." Methods Mol. Biol.: Protein Engineering Protocols pp127-154 (2007)

Accompanying Excel worksheets

28. J. Volpato, E. Fossati and J. N. Pelletier "Increasing methotrexate resistance by combination of active-site mutations in human dihydrofolate reductase." J. Mol. Biol., 373, 599-611 (2007)

27. N. Doucet and J. N. Pelletier "Simulated annealing exploration of an active-site tyrosine in TEM-1 ß-lactamase suggests the existence of alternate conformations." Proteins, 69, 340-348 (2007)

26. N. Doucet, P.-Y. Savard, J. N. Pelletier and S. M. Gagné "NMR investigation of Tyr-105 mutants in TEM-1 ß-lactamase: Dynamics are correlated with function." J. Biol. Chem., 282, 21448-21459 (2007)

25. J. N. Pelletier and Robert Lortie "Sequence activity relationships guide directed evolution" Nat. Biotechnol., 25, 297-298 (2007)

2006

24. J. Puglia, T. Wang, C. Smith-Snyder, M. Cote, M. Scher, J. N. Pelletier, S. John, C. B. Jonsson and M. J. Roth. "Revealing Domain Structure through Linker-Scanning Analysis of the Murine Leukemia Virus (MuLV) RNase H and MuLV and Human Immunodeficiency Virus Type 1 Integrase Proteins." J. Virol., 80, 9497-9510 (2006)

23. A. Nisole, F.-X. Lussier, K. L. Morley, F. Shareck, R. J. Kazlauskas, C. Dupont and J. N. Pelletier. "Extracellular Production of Streptomyces lividans Acetyl-xylan Esterase A in Escherichia coli for Rapid Detection of Activity." Protein Expr. Purif., 46, 274-284 (2006)

2005

22. S. M. F. G. Gillet, J. N. Pelletier and J. W. Keillor. "A Direct Fluorometric Assay for Tissue Transglutaminase." Anal. Biochem., 347, 221-226 (2005)

21. R. A. Chica*, N. Doucet* and J. N. Pelletier. "Engineering enzyme activity by semi-rational approaches: combining the benefits of directed evolution and rational design." Curr. Opin. Biotechnol., 16, 378-384 (2005) (*Equal contribution)

20. L. Berrou, Y. Dodier, A. Raybaud, A. Tousignant, O. Dafi, J. N. Pelletier and L. Parent. "The C-terminal Residues in the Alpha-interacting Domain (AID) Helix Anchor CaV {beta} Subunit Interaction and Modulation of CaV2.3 Channels." J. Biol. Chem., 280, 494-505 (2005)

2004

19. A.R. Schmitzer, F. Lépine and J. N. Pelletier. "Combinatorial exploration of the catalytic site of a drug-resistant dihydrofolate reductase: creating alternative functional configurations." Protein Eng. Des. Sel., 11, 17, 809-819 (2004)

Cover illustration:

18. N. Doucet, P.-Y. De Wals and J. N. Pelletier. "Site-saturation mutagenesis of Tyr105 reveals its importance in substrate stabilization and discrimination in TEM-1 ß-lactamase." J. Biol. Chem., 279, 46295-46303 (2004)

17. A. Tousignant and J. N. Pelletier. "Protein motions promote catalysis." Chemistry & Biology, 11, 1037-1042 (2004)

16. R. A. Chica, P. Gagnon, J. W. Keillor and J. N. Pelletier. "Tissue transglutaminase acylation: Proposed role of conserved active site Tyr and Trp residues revealed by molecular modelling of peptide substrate binding." Protein Science, 13, 979-991 (2004)

15. S. M. F. G. Gillet, R. A. Chica, J. W. Keillor and J. N. Pelletier. "Expression and rapid purification of highly active hexahistidine-tagged guinea pig liver transglutaminase." Protein Expr. Purif., 33, 256-264 (2004)

2002

14. K. M. Arndt, J. N. Pelletier, K. M. Muller, A. Pluckthun and T. Alber. "Comparison of In Vivo Selection and Rational Design of Heterodimeric Coiled Coils." Structure 10, 1235-1248 (2002)

13. P. Amstutz, J. N. Pelletier, A. Guggisberg, L. Jermutus, S. Cesaro-Tadic, C. Zahnd and A. Plückthun. "In vitro selection for catalytic activity with ribosome display." J. Am. Chem. Soc. 124(32), 9396-403 (2002)

2001

12. J. N. Pelletier and S. Sidhu. "Mapping protein-protein interactions with combinatorial biology methods" Curr. Opin. Biotechnol. 12, 340-347 (2001)

11. L. Jermutus and J. N. Pelletier. "Creating and evaluating protein diversity" Curr. Opin. Biotechnol. 12, 331-333 (2001)

10. J. N. Pelletier. "A RACHITT for our toolbox" Nature Biotech. 19, 314-315 (2001)

2000

09. K. M. Arndt, J. N. Pelletier, K. M. Müller, T. Alber, S. W. Michnick and A. Plückthun. "A heterodimeric coiled-coil pair selected in vivo from a designed library-vs-library ensemble." J. Mol. Biol. 295, 627-639 (2000)

1999

08. J. N. Pelletier, K.M. Arndt, A. Plückthun and S.W. Michnick. "An in vivo library-vs-library selection of optimized protein-protein interactions." Nature Biotech. 17:683-690 (1999)

07. J. N. Pelletier, I. Remy and S. W. Michnick. "Protein-fragment complementation assays: A general strategy for in vivo detection of protein-protein interactions." J. Biomol. Tech. 10: 32-40 (1999)

1998

06. J. N. Pelletier, F.-X. Campbell-Valois and S. W. Michnick. "Oligomerization domain-directed reassembly of active dihydrofolate reductase from rationally designed fragments." Proc. Nat. Acad. Sci. USA 95: 12141-12146 (1998)

1997

05. J. N. Pelletier and S. W. Michnick. "A Strategy for Detecting Protein-Protein Interactions in vivo Based on Protein Fragment Complementation." Protein Engineering 10 supp: 89 (1997)

04. M. Allaire, Y. Li, N. Mejia, J. N. Pelletier, R. E. MacKenzie and M. Cygler. "Crystallization of the Bifunctional Methylenetetrahydrofolate Dehydrogenase/ Methenyltetrahydrofolate Cyclohydrolase Domain of the Human Trifunctional Enzyme." Proteins, 26:479-480 (1997)

1996

03. J. N. Pelletier and R. E. MacKenzie. "Methenyltetrahydrofolate Cyclohydrolase Catalyzes the Synthesis of (6S)-5-Formyltetrahydrofolate." Bioorganic Chemistry, 24: 220-228 (1996)

1995

02. J. N. Pelletier and R. E. MacKenzie. "Binding and Interconversion of Tetrahydrofolates at a Single Site in the Bifunctional Methylenetetrahydrofolate Dehydrogenase/ Cyclohydrolase." Biochemistry, 34: 12673-12680 (1995)

1994

01. J. N. Pelletier and R.E. MacKenzie. "Binding to the 2',5'-ADP Subsite Stimulates Cyclohydrolase Activity of Human NADP+-dependent Methylenetetrahydrofolate Dehydrogenase/ Cyclohydrolase." Biochemistry, 33:1900-1906 (1994)

Book chapters

03. M. Denault and J. N. Pelletier "Protein library design and screening: Working out the probabilities". Methods in Molecular Biology: Protein Engineering Protocols., Humana Press (2007)

02. I. Remy, J. N. Pelletier, A. Galarneau and S. W. Michnick. "Dynamic visualisation and library screening of protein interactions in living cells with protein fragment complementation strategies". Protein-protein interactions: A molecular cloning laboratory manual, J. Sambrook, ed., Cold Spring Harbour Laboratory Press (2001)

01. S. W. Michnick, I. Remy, F.-X. Campbell-Valois, A. Vallée-Bélisle and J. N. Pelletier. Detection of protein-protein interactions with protein fragment complementation strategies. Methods in Enzymology, 328, 208-230 (2000)